Peptide Dosage Calculator (Reconstitution)
Peptide dosing is a delicate and complicated art. We’ve created a peptide dosage calculator to help you reconstitute your peptides fresh from the source.
Medically reviewed by Dr. Mohammed Fouda
Dr. Fouda uses his extensive knowledge and expertise as an academic scholar to medically review articles for scientific accuracy and credibility, ensuring that the medical information presented is up-to-date and trustworthy. He is responsible for ensuring the quality of the medical information presented on our website.
- Updated On March 8, 2024
Reconstitution and Dosage Calculator
Peptides – the protein’s little brother – can do wonders for our bodies! They can manage our circadian rhythm (1), regulate growth hormones (2), help with weight loss (3), and even make us look younger (4).
Now, before you start flexing your peptide muscles, you need to learn the proper way to reconstitute them. Apparently, a lot of people struggle with this step. Okay, we made up that statistic, but it’s probably close to the truth. The struggle is real, people.
But don’t worry, we’re here to help you navigate this tricky process. It’s like a chemistry experiment, but instead of blowing things up, you’ll be improving your health. Plus, you’ll feel like a mad scientist, and who doesn’t love that feeling?
So, if you want to master your peptide reconstitution, follow our guide step-by-step, and soon you’ll be a reconstitution pro. No more confusion or frustration, just pure peptide power!
How to use The Calculator
While you can go on to use the conventionally manual way to reconstitute peptides, it gets a whole lot easier to use the reconstitution calculator, and get the right dosage!
With our easy-to-use reconstitution calculator, you now don’t have to worry about all the multiplication, division, or unit conversion. All you have to do is just follow the easy steps and BOOM!
The very first step is to pick the syringe size that you have purchased. It would ideally be either a 0.3ml, 0.5ml, or 1ml syringe.
The next step is to add the mass of the peptide in mg that you want to reconstitute (This is generally going to be listed on the peptide vial) with the βx’ ml of bacteriostatic water in order to get your desirable dosage in mcg.
Once you have put the details, our reconstitution calculator will use its algorithm and devise the volume needed to get your peptides reconstituted!
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Make sure you use Bacteriostatic Water
Bacteriostatic water and sterile water are essentially used to dissolve drugs/medications for injection purposes.
Bacteriostatic water is used to dilute or dissolve medications intended for aqueous injection. Its preservative agent (0.9% benzyl alcohol) prevents the growth of bacteria and therefore, extends the shelf life for up to 28 days and enables the withdrawal of multiple doses from a single vial without contamination (5).
On the other hand, a sterile water vial doesn’t contain any preservatives/additives which can keep it free of pathogens for a longer time, thus one will have to use it immediately after opening the seal.
So, if you are someone who has seen the vials of both types of water, I am sure you can now understand why a bacteriostatic water vial contains considerable volume while a sterile water vial contains just a few milliliters.
Despite the favorable safety profile of bacteriostatic water, clinicians caution against its use in pregnant women, neonates, and certain medications. It is also unsafe for injection without a solvent and is contraindicated for intrathecal injections (5).
How to Store Peptides
Peptides should be stored in a dry, cool, dark place. For best preservation, store at 4Β°C or colder away from bright light. Dry peptides are stable at room temperature for days to weeks but for long-term storage -20Β°C is to be preferred (6).
Contamination with moisture will greatly decrease the long-term stability of solid peptides. A vial containing a peptide should be allowed to warm to room temperature before being opened. After removing the desired quantity, the vial should be re-sealed, preferably under an atmosphere of dry inert gas, and then returned to cold storage (6). An ideal container for peptide manipulation should be clean, chemically inert, optically clear, strong, and available in an appropriate size.
Glass and plastic vials are generally satisfactory for the purpose, however, care does need to be taken with plastic vials when organic solvents are to be used (6). Polypropylene vials are both strong and chemically inert but if high visibility is required glass is a better option. Please appreciate that peptide in solution can and do adsorb to many materials. This may occur to varying extents being dependent upon factors such as constituent amino acids, vial material, and peptide concentration.
At high dilution, it is possible to lose large percentages of peptide due to adsorption to surfaces thereby grossly distorting subsequent results. The use of high-quality specialist glass and polypropylene vials can lessen this problem (6). The shelf life of peptide solutions is limited especially for peptides containing C, M, N, Q, and W. To prolong the storage life of peptides in solution, sterile buffers with a pH of around 5-6 should be used. Aliquots should be stored at -20Β°C or colder wherever possible.
Avoid the use of frost-free freezers, which vary enormously in temperature during the frequent automatic defrosting cycles. Repeated freeze-thaw cycles are deleterious to peptides (6).
All Peptide Guides
Still Have Questions?
Drop us a comment down below and one of our expert coaches will respond to it, you can also email is at [email protected].
How does the math work when reconstituting a peptide with 5mg of two different peptides in the same vial? Example would be 5g of CJC-1295 and 5g of ipamorelin. if a dosage is say 20mcg of each individually, wht is a dosage of the mix?
did you ever get an answer? I have this same question…. Please let me know if you get an answer. Need to know how much to buy
Iβm looking for the same answer.
I’m pretty sure it is still only considered a total of 5mg you have in one vial.
That’s really simple. If you have a 50/50 mix in the same vial, you would withdraw a TOTAL of 40 MCG into the syringe, since there is an equal amount of each peptide in the mix you would be getting 20 MCG of each product. 20+20=40
If I have a 2mg bottle how much water total do I put into the vial?
That is AOD
How do I convert mcg to units.
It depends on the compound, there is no universal conversion
Where is the calculator?
Where is the calculator? I canβt find it
It’s fixed now!
How many Days worth does this reconstitute to? If I have a 5mg mass that I need to draw 300mcg doses daily, it tells me the volume to draw based on how many ML of water I use but it doesn’t tell me the quantity of doses that will yield
If you convert 5mg to mcg, it is 5000mcg. So divide 5000mcg by 300mcg.
What is considered “short term” with regards to storage? The vial I am reconstituting will probably last 8-10 weeks. Is that still short term?
It depends on the active compound within
I’m trying to reconstitute 5mg of trizeatide w 3ml of bacteriasc water.
Trying to figure out the mixture for 1 /wk dosage of .50mg.
How much water do I mix w the 5mg trizeatide? And how many dosages will I get out of one 5ml vial?
You just mentioned a 5mg vial and a 5ml vial. Those are not the same, please clarify
GM Amber. I am doing the same thing. Did you get a response
You can mix as much, (or as little) water as your vial will hold. You will just be withdrawing less or more product out of the vial to get the dosage you want. But no matter how much water you choose to use, you would get ten 0.5mg shots out of your 5 mg vial. 10×0.5mg=5mg. (The calculator at the top of the page is a great tool.)
But where do you get the 10 from? I am so confused. The calculator above is great but I can’t figure out how long a vial will last me at a particular dose.
Example: I entered in 1ml syringe, 10mg powder, 3ml bac water and a 250mcg dose.It tells me where I need to measure my syringe at. But how do I figure out how many doses I have at that measurement?
how long will one reconstituted vial of tirzepatide last in the frige
Peptides usually only last a couple of days / weeks once reconstituted
The calculator doesn’t work for custom doses
Actually, yes it does! Look at it again and you see the boxes under each option that says “OTHER”. Simply click on that box and fill in what ever numbers you want.
What is the dosage for tesomorelin
You can read more about that compound here: https://muscleandbrawn.com/peptides/tesamorelin/
How do I figure out how many doses are in a vial at the selected measurements I entered? Example: I entered in 1ml syringe, 10mg powder, 3ml bac water and a 250mcg dose.It tells me where I need to measure my syringe at. But how do I figure out how many doses I have at that measurement?
10,000/250 = 40
How much bacteriostatic water is needed to reconstitute 5mg of Tirzepatide peptide to achieve a 100unit (10mg) dose?
I need this answer
… 5mg < 10mg. You could use 10,000L and 5 would still be smaller than 10
you can’t magically turn 5mg into 10mg, no matter how much BAC you add.
1ml
How much water do I add to 5mg Tirzepatide
I need to know your dose
I would like to know .5mg dose a week would be. If you have the 5mg of tirzepatide, how much water. If that makes sense
You can use our calculator for this π
How would you go about dosing a blend of tesamoraline, cjc 1295, and ipamoraline?
You can schedule a call with me here https://muscleandbrawn.com/coaching/
How Much Bacteriostatic Water do I add to 5mg of DSIp? I am a 57 year old male about 27LBS?
I reconstitute 2ml of Bacteriostatic into a 5mg peptide.
If I purchase a 10mg peptide and reconstitute with 4ml will it have the same effect?
Thanks
Yes! You would withdraw the same amount into the syringe that you would do with the 5m vial. You can double check it with the handy calculator at the very top of this page.
I am confused about how much water to use when mixing. Also do you keep it refrigerated between use?
You can use any amount of water, the dosage per unit just decreases. And yes, you have to refrigerate them
I don’t u derstand how every vial is advised to mix with 2ml. Rhe 10mg, 5mg and 2mg.
I have a 10 mg vial of TB500. How much Bariostatic water do I use to reconstitute? I will be using 7.5 mg / week in 2 equal doses on Tuesday and Thursday.
I got 100mg of GHK-Cu in a single vial (a 5mL vial I believe). The dry powder takes up about 60% of the volume of the vial. Do I just reconstitute in this same vial? Or can I split the powder into 2 vials somehow? Even if I’m able to reconstitute in this single vial, am I then not obligated to consume the entire vial within 30 days due to BAC degradation?
How long and often do I take BPC 157?
https://muscleandbrawn.com/peptides/bpc-157/
I JUST RECEIVED SOME GHK-CU THAT SHOWS 50MCG ON THE BOTTLE?
Do I add in enough bag water to fill the small bottle the powder is in?
And the dosage shows .2ml how is that figured or is that equal to 20mg?
I am reconstituting GHK-Cu (100mg) into a 10 ml nasal spray. I don’t know how to work out the dosage. Any help would be greatly appreciated.
When using the calculator, if the vial is a mix of 5mg/5mg – is it correct to assume the vial contains 10mg total volume? So set the vial calculation to 10 and NOT 5?
Yes
I have the peptides in powder form in small vials. I have the bacteriostatic water (3ml) to add to the powder. The vial the peptide comes in is too small to add the water. I also have bigger vials to add the peptide powder to first, then the water? If so how do I get the tops off both vials.
I’m not quite getting it just yet. I could use a suggestion please.
I have 5mg vials of ipamorelin and my 3ml bottles of bacteriostatc water. I am comfortable doing the reconstitution process, however I do not know how much water to place into the ipamorelin.
Thank you for any assistance you can offer.
David
It doesn’t matter. Use less and the concentration is higher, more and the concentration is lower
I am interested in the Humanin peptide but I am unable to locate a dose for research purposes. Any suggestions?
Thanks
Keith
Just confirming that I am mixing this correctly:
6mg vial of powder mixed with 2ml of water to get a 15mg dosage I would pull 20units in a 1ml syringe.
Very incorrect
I have a 10mg small vial of BPC157. The desired dose is 200mcg they sent a 20ml vial of bacteriostatic water with the order. Iβm thinking only about 2ml will fit in the vial of peptide. When using the calculator is the volume at the bottom the amount you draw up in the syringe?
Iβm pretty good with math but this all still baffles me. I have a 5 mg bottle of semeglitude.. my goal is a 5mg dose. I will add 3ml bac water . I am using 1 ml syringe. To obtain 5 mg dose how much will I draw up of the solution ? Thank you!
You have a 5mg bottle and you want a 5mg dose, you’d draw all of it. Are you sure your numbers are correct?
All of it. If the contents of your bottle is equal to your desired dose then you need to draw up all of it, regardless of how much bac water you add. Are you sure you require a 5mg dose?
just received 10mg GLP-1 and 30ml bacteriostatic water. Want to start at .25mg peptide for first few weeks, then get to .5mg peptide. Do I reconstitute all 10mg at one time, or dose by dose? How long will reconstituted peptide last? How long will unreconstituted peptide last? How long will bacteriostatic water last? Do I need to use bacteriostatic water? Or can I just use .25mg peptide to start?
This is confusing.
Thanks so much!
Also syringe size is 1ml
Hey guys. I had many of the same questions. I decided to ask AI and verify with Google for more details. I’ve got some screenshots of my questions and answers. Hope it helps. I just started today. IPAMORELIN, TB-500, & BPC-157.
See imagine
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I have 10mg of Tirzepatide and taking 7.5 weekly. Do I reconstitute with 10ml/units or still continue with 5ml/units and take a smaller dose?
I have 1mg of IGF1-LR3.
Need 50mcg/day
How much bacteriostatic water do I add to get 50mg daily?