Peptide Dosage Calculator + Chart + Timing (Reconstitution)
Peptide dosing is a delicabte and complicated art. We’ve created a peptide dosage calculator to help you reconstitute your peptides fresh from the source, and then a peptide dosage chart with some common guidelines on dosing, timing, and injection site with all our sources and citations.
Medically reviewed by Dr. Mohammed Fouda
Dr. Fouda uses his extensive knowledge and expertise as an academic scholar to medically review articles for scientific accuracy and credibility, ensuring that the medical information presented is up-to-date and trustworthy. He is responsible for ensuring the quality of the medical information presented on our website.
- Updated On September 20, 2023
Reconstitution and Dosage Calculator
Peptides – the protein’s little brother – can do wonders for our bodies! They can manage our circadian rhythm (1), regulate growth hormones (2), help with weight loss (3), and even make us look younger (4).
Now, before you start flexing your peptide muscles, you need to learn the proper way to reconstitute them. Apparently, a lot of people struggle with this step. Okay, we made up that statistic, but it’s probably close to the truth. The struggle is real, people.
But don’t worry, we’re here to help you navigate this tricky process. It’s like a chemistry experiment, but instead of blowing things up, you’ll be improving your health. Plus, you’ll feel like a mad scientist, and who doesn’t love that feeling?
So, if you want to master your peptide reconstitution, follow our guide step-by-step, and soon you’ll be a reconstitution pro. No more confusion or frustration, just pure peptide power!
To go to the complete dosage chart click here.
How to use The Calculator
While you can go on to use the conventionally manual way to reconstitute peptides, it gets a whole lot easier to use the reconstitution calculator, and get the right dosage!
With our easy-to-use reconstitution calculator, you now don’t have to worry about all the multiplication, division, or unit conversion. All you have to do is just follow the easy steps and BOOM!
The very first step is to pick the syringe size that you have purchased. It would ideally be either a 0.3ml, 0.5ml, or 1ml syringe.
The next step is to add the mass of the peptide in mg that you want to reconstitute (This is generally going to be listed on the peptide vial) with the ‘x’ ml of bacteriostatic water in order to get your desirable dosage in mcg.
Once you have put the details, our reconstitution calculator will use its algorithm and devise the volume needed to get your peptides reconstituted!
Warning: The content on Muscleandbrawn.com and the information included in this article is intended for entertainment and informational purposes only. It is not intended nor implied to be a substitute for professional medical advice. Prior to buying anything, check that it is compliant where you live with your current government laws. We frequently mention research chemicals that are not made for human consumption. Therefore, before purchasing any product for personal use, consult with your doctor or healthcare provider first.
Make sure you use Bacteriostatic Water
Bacteriostatic water and sterile water are essentially used to dissolve drugs/medications for injection purposes.
Bacteriostatic water is used to dilute or dissolve medications intended for aqueous injection. Its preservative agent (0.9% benzyl alcohol) prevents the growth of bacteria and therefore, extends the shelf life for up to 28 days and enables the withdrawal of multiple doses from a single vial without contamination (5).
On the other hand, a sterile water vial doesn’t contain any preservatives/additives which can keep it free of pathogens for a longer time, thus one will have to use it immediately after opening the seal.
So, if you are someone who has seen the vials of both types of water, I am sure you can now understand why a bacteriostatic water vial contains considerable volume while a sterile water vial contains just a few milliliters.
Despite the favorable safety profile of bacteriostatic water, clinicians caution against its use in pregnant women, neonates, and certain medications. It is also unsafe for injection without a solvent and is contraindicated for intrathecal injections (5).
Where to Buy Syringes and Bacteriostatic Water?
We use 30 gauge 5/16th of an inch (8mm) for our subcutaneous injections, but we recently found a source that sells:
- Injection syringes
- Bacteriostatic Water
- Drawing syringes (To draw the water and insert them into the aliquots)
- Alcohol wipes
ALL IN ONE BOX! That’s right, no more shopping around. Just order it all from them, and save some money while you’re at it.
Introducing…..
Peptide Research Injection Kit
Introducing the all-inclusive Concierge Research Injection Kit, designed to streamline the research process by providing everything needed for reconstitution, storage, and administration of substances in a single package. Simplify the workflow and eliminate the hassle of sourcing materials from multiple vendors.
This comprehensive kit includes:
– Bacteriostatic Water (30mL): Ideal for diluting and reconstituting substances while maintaining a sterile, bacteria-free environment.
– Insulin Syringes (0.5 cc/mL x 29g x ½): Precision-engineered syringes for accurate administration, featuring fine gauge needles and a 0.5 cc/mL capacity.
– Alcohol Prep Pads: Ensure cleanliness and minimize contamination with these pre-moistened pads for sterilizing injection sites and vials.
– Sterile Empty Glass Vial (10mL): A contamination-free container for safely storing reconstituted substances.
– Large Needles + Syringes Combo (3cc x 21g x 1): Versatile, larger-capacity syringes with thicker gauge needles for injecting Bac water into the peptide vial.
With this Concierge Research Injection Kit, researchers can focus on obtaining results and analyzing data more efficiently. Say goodbye to wasted time and effort in sourcing research materials and embrace a seamless research experience.
Introducing…..
Peptide Research Injection Kit
Introducing the all-inclusive Concierge Research Injection Kit, designed to streamline the research process by providing everything needed for reconstitution, storage, and administration of substances in a single package. Simplify the workflow and eliminate the hassle of sourcing materials from multiple vendors.
This comprehensive kit includes:
– Bacteriostatic Water (30mL): Ideal for diluting and reconstituting substances while maintaining a sterile, bacteria-free environment.
– Insulin Syringes (0.5 cc/mL x 29g x ½): Precision-engineered syringes for accurate administration, featuring fine gauge needles and a 0.5 cc/mL capacity.
– Alcohol Prep Pads: Ensure cleanliness and minimize contamination with these pre-moistened pads for sterilizing injection sites and vials.
– Sterile Empty Glass Vial (10mL): A contamination-free container for safely storing reconstituted substances.
– Large Needles + Syringes Combo (3cc x 21g x 1): Versatile, larger-capacity syringes with thicker gauge needles for injecting Bac water into the peptide vial.
With this Concierge Research Injection Kit, researchers can focus on obtaining results and analyzing data more efficiently. Say goodbye to wasted time and effort in sourcing research materials and embrace a seamless research experience.
How to Store Peptides
Peptides should be stored in a dry, cool, dark place. For best preservation, store at 4°C or colder away from bright light. Dry peptides are stable at room temperature for days to weeks but for long-term storage -20°C is to be preferred (6).
Contamination with moisture will greatly decrease the long-term stability of solid peptides. A vial containing a peptide should be allowed to warm to room temperature before being opened. After removing the desired quantity, the vial should be re-sealed, preferably under an atmosphere of dry inert gas, and then returned to cold storage (6). An ideal container for peptide manipulation should be clean, chemically inert, optically clear, strong, and available in an appropriate size.
Glass and plastic vials are generally satisfactory for the purpose, however, care does need to be taken with plastic vials when organic solvents are to be used (6). Polypropylene vials are both strong and chemically inert but if high visibility is required glass is a better option. Please appreciate that peptide in solution can and do adsorb to many materials. This may occur to varying extents being dependent upon factors such as constituent amino acids, vial material, and peptide concentration.
At high dilution, it is possible to lose large percentages of peptide due to adsorption to surfaces thereby grossly distorting subsequent results. The use of high-quality specialist glass and polypropylene vials can lessen this problem (6). The shelf life of peptide solutions is limited especially for peptides containing C, M, N, Q, and W. To prolong the storage life of peptides in solution, sterile buffers with a pH of around 5-6 should be used. Aliquots should be stored at -20°C or colder wherever possible.
Avoid the use of frost-free freezers, which vary enormously in temperature during the frequent automatic defrosting cycles. Repeated freeze-thaw cycles are deleterious to peptides (6).
Peptide Dosage Chart with Timing, Injection Location, and Best Stacks
We spent 20+ hours reading research papers, perusing clinical websites and browsing research peptide brands to come up with the ULTIMATE express guide to current up-to-date medical literature on peptide usage, dosage, cycles timing, indications, forms, and studies.
For first time peptide users we know its daunting and intimidating, hopefully this gives you a better idea of what the f*** is going on with most peptides, but remember to exercise caution and consult with your doctor prior to any usage.
Below the individual peptides we’ve added sections on stacks, and we’ve also explained what all the columns mean below. If you want to learn more about an individual peptides click on it or view all our peptide guides below the peptide chart!
The Complications of Dosing Peptides
While there is no fixed dosage of peptides and each peptide accounts for a different dose.
Recommended dosages are usually based on either clinical trials or users’ experience.
Therefore, if you decided to use any peptide for personal purposes, it is crucial to consult with your doctor/healthcare provider first.
For example, the recommended dosage of BPC-157 is 1mcg to 10mcg per kg of body weight which accounts for 200mcg to 1000mcg per injection. But note that BPC-157 is not approved by the FDA for human use.
On the other hand, TB-500, another potential peptide for quick healing is usually dosed at 7.66mg per week. However, as BPC-157, TB-500 is not approved by the FDA for human use.
So it all differs and the exact dosage can only be prescribed by your healthcare provider.
Scroll left and right to see the full chart if your screen is not big enough
| Peptides | 💊 Studied Dosage | 💉 Injection Site | ⏳️ Typical Cycle Length | 🕑 Timing of Dose | ✅ Use/Indication | 🛒 Forms | 📚 Recent Studies |
| Click on peptide for full breakdown | *In accordance with the limited clinical trials and Reddit discussions* | *In accordance with the limited clinical trials and Reddit discussions* | *Based purely on user experiences* | *In accordance with the limited clinical trials and Reddit discussions* | *Based on what Peptide Vendors offer* | *Pubmed and Google Scholar* | |
| Individual Peptides | |||||||
| A | |||||||
| Anamorelin | 100 mcg/day | Not Applicable | 12 Weeks | Before Meals | Cancer Cachexia, Appetite | Oral | [1] [2] [3] |
| ARA 290 | 5 mg/day | Subcutaneous (multiple skin sites) | 4-5 Weeks | Variable | Neuropathic Pain | Injectable | [1] [2] |
| AOD 9604 | 300 mcg/day | Subcutaneous (multiple skin sites) | 20 Days | In Morning- 30 mins prior to eating | Body Fat | Powders, Spray | [1] [2] |
| B | |||||||
| BPC-157 | 1-10 mcg/kg of bodyweight | Subcutaneous, IM | 4-12 Weeks | Before meal and before bed | Anti-inflammatory, Wound healing | Capsules, Injections, Sublingual and dermal Patches | [1] [2] [3] |
| C | |||||||
| Cerebrolysin | Depends on the purpose of intake | IM, IV | Variable | No Specific Timing | Senile/Vascular Dementia, Cerebrovascular disorder | Injectable | [1] [2] |
| CJC 1295 | 30-60 mcg/day | Subcutaneous (multiple skin sites) | 12 Weeks | Before bed- 2 hrs after last meal | Muscle growth, Fat loss | Injectable | [1] [2] |
| D | |||||||
| DSIP | 100-200 mcg/day | Subcutaneously, IV, IM | Variable | 1-3 hours before bed | Irregular Circadian Rhythm, Insomnia | Powder | [1] [2] [3] |
| E | |||||||
| Epitalon | 5-10 mg | Subcutaneously, IV, IM | 2-3 Weeks | Morning- 30 mins prior to eating | Anti-Aging | Capsule, Injectable Nasal Spray | [1] [2] [3] |
| F | |||||||
| Follistatin 344 | 50-100 mcg/day | Subcutaneously, IV, IM | 10-30 days | No Specific Timing | Muscle Growth, Endurance | Powder, Liquid | [1] [2] [3] |
| G | |||||||
| GHK-CU | 0.2 ml/day | Subcutaneous (multiple skin sites) | Variable | No Specific Timing | Wound Healing, Anti-inflammatory | Injectable, Transdermal | [1] [2] |
| GHRP-2 | 150-300 mcg/day | Subcutaneous(Abdomen) | 12-14 Weeks | An hour before Meal | Muscle Growth, Rapid Healing | Injectable, Capsules | [1] [2] [3] |
| GHRP-6 | 100-150 mcg/day | Subcutaneous(Abdomen) | 12-14 Weeks | 30 Minutes Before Meal | Muscle Growth, Rapid Healing | Injectable, Capsules | [1] [2] |
| H | |||||||
| Hexarelin | 200-300 mcg/day | Subcutaneous(Abdomen) | 12-16 Weeks | Before Meals | Muscle building, Fat loss, rapid recovery | Injectable, Tablets | [1] [2] |
| HGH Frag | 200 mcg/day | Subcutaneous, IV | 4-8 Weeks | Before Workout | Obesity, Muscle Mass | Injectable, Tablets | [1] [2] [3] |
| I | |||||||
| IGF-1 | 20-100 mcg/day | Subcutaneous (multiple skin sites) | 4 Weeks | After Workout | Lean Muscle Mass, Fat loss | Injectable | [1] [2] [3] |
| IGF-1 LR3 | 20-40 mcg/day | Subcutaneous (multiple skin sites) | 4 Weeks | Before Workout | Muscle Mass, Bodybuilding | Injectable | [1] [2] [3] |
| Ipamorelin | 200 mcg/day | Subcutaneous, IM | 60-90 Days | Before bed- 2 hrs after last meal | Muscle growth, Fat loss | Injectable | [1] [2] [3] |
| K | |||||||
| KPV | 200-400 mcg/day | Subcutaneous (multiple skin sites) | 4 Weeks | No Specific Timing | Anti-inflammatory, Wound healing | Injectable, Oral Sprays, Creams, Capsules | [1] [2] |
| Kisspeptin 10 | 5-10 mg/day | IV | 4 Months | No Specific Timing | Infertility | Injectable, Capsules | [1] [2] [3] |
| L | |||||||
| Ll-37 | 100 mcg/day | Subcutaneous (multiple skin sites) | 2-4 Weeks | No Specific Timing | Anti-microbial | Injectable | [1] [2] |
| Lenomorelin | 7.5 mcg/kg of body weight | Subcutaneous (multiple skin sites) | 10-12 Weeks | Before Meal | Appetite, Strength | Injectable | [1] [2] [3] |
| M | |||||||
| Macimorelin | 0.5 mg/kg of body weight | Not Applicable | Usually a single dose | 8 hours of fasting before use | Fat loss, Muscle Mass | Oral Granules | [1] [2] [3] |
| Melanotan I | 0.25-2 mg/day | Subcutaneous, IM | Variable | No Specific Timing | Skin tanning, Erythropoetic Protoporphyria | Injectable, Nasal Sprays | [1] [2] |
| Melanotan II | 0.25-2 mg/day | Subcutaneous, IM | Variable | No Specific Timing | Erectile Dysfunction & Skin Tanning | Injectable, Nasal Sprays | [1] [2] [3] |
| Mots-c | 1ml/thrice weekly | Subcutaneous (multiple skin sites) | 4 Weeks | No Specific Timing | Metabolic Felxibility, Osteroporosis | Injectable | [1] [2] |
| MGF | 200 mcg/day | Subcutaneous (multiple skin sites) | 4 Weeks | Before Workout | Muscles growth, Tissue Repair | Injectable | [1] [2] |
| P | |||||||
| Peg MGF | 5 mg/day | Subcutaneous (multiple skin sites) | 4 Weeks | No Specific Timing | Endurance, Boosts Immune System | Injectable | [1] |
| Pentosan | 100-250 mg/day | IM, Intra-auricular | 1-6 Months | 1-2 hrs after Meals | Cardiovascular Complications, Joint Pain | Injectable, Capsules, Oral Suspensions | [1] [2] [3] |
| PTD-DMB | 30 ml/day | Not Applicable | Variable | No Specific Timing | Hair loss, Hair Regeneration | Hair Sprays | [1] |
| PT-141 | 1-2 mg | Subcutaneous (multiple skin sites) | 8 times/month | 1-5 hrs before intercourse | Libido & Erectile dysfunction | Injectable, Nasal Sprays, Pills | [1] [2] [3] |
| R | |||||||
| RU 58841 | 50 mg/day | Not Applicable | Variable | After Showering- On dry hair | Hair Loss, Male Pattern Baldness | Powder, Liquid (Topical application) | [1] [2] [3] |
| S | |||||||
| Semaglutide | 0.25 mg/week | Subcutaneous (multipe skin sites) | 4 Weeks | No Specific Timing | Weight Loss, Appetite | Injectable | [1] [2] [3] |
| Sermorelin | 10-20 mcg/kg of body weight | Subcutaneous, IV | 3-6 Months | Before bed | Muscle Mass, Fat Loss | Injectable | [1] [2] [3] |
| Semax | 0.3-1 ml/twice weekly | Subcutaneous (multipe skin sites) | Variable | No Specific Timing | Neuroprotective, Stress-protective | Injectable | [1] [2] [3] |
| T | |||||||
| TB 500 | 7.6 mg/week | IM, IV, Subcutaneous | 2-6 Weeks | Once a Week | Anti-inflammatory & Muscle Growth | Capsules, Injectable, Patches | [1] [2] [3] |
| Tesamorelin | 2 mg/day | Subcutaneous (multipe skin sites) | 2 Months | Before bed- 2 hrs after last meal | HIV- lipodystrophy | Injectable | [1] [2] [3] |
| Thymosin Alpha-1 | 0.8-6.4 mg/twice weekly | Subcutaneous (multipe skin sites) | 6-12 Months | No Specific Timing | Immunocomproised disorders, autoimmune diseases | Injectable, Nasal Sprays | [1] [2] [3] |
| Thymosin Beta-4 | 2-12 mg/day | IM, Subcutaneous | 3-6 Months | Injected close to the site of injury | Wound healing, Anti-inflammatory | Powder, Injectable | [1] [2] [3] |
| V | |||||||
| Vasoactive Intestinal Peptide | Depends on the purpose of intake | IV, Intracavernously | Variable | No Specific Timing | Anti-inflammatory, COPD, Pulmonary Arterial HTN | Injectable | [1] [2] [3] |
| Z | |||||||
| Zn-Thymulin | 1 ml/day | Subcutaneous, IM | Variable | At Night | Hair Loss, Hair pigmentation | Topical Use | [1] [2] |
| Peptide Stacks | |||||||
| BPC-157= 500 mcg/day, TB-500= 5 mg/twice weekly | Subcutaneous, IM | 4 Weeks | Before Meal | Healing, Protein Formation, Muscle Mass | Injectable | ||
| Ipamorelin and CJC-1295 | Ipamorelin= 150mcg/day, CJC-1295= 150mcg/day | Subcutaneous (multiple skin sites) | 4 Weeks | Before Bed | Muscles Growth, Fat loss | Injectable | |
| Melanotan I and Melanotan ll | Melanotan l= 0.25-2 mg/day, Melanotan ll= 0.25-2 mg/day | Subcutaneous, IM | Variable | No Specific Timing | Skin Tanning, Erectile Dysfunction | Injectable, Nasal Sprays | |
| Ipamorelin and Sermorelin | Ipamorelin= 200-300mcg/day, Sermorelin= 200 mcg/day | Subcutaneous (multiple skin sites) | 2-3 Months | Before Bed | Muscles Growth, Fat loss | Injectable | |
| GHRP-6, Sermorelin | GHRP-6= 100 mcg/day, Sermorelin= 100 mcg/day | Subcutaneous(Abdomen) | 3 Months | Before Meal | Fat burn, Endurance, Muscle Mass | Injectable | |
| GHRP6 and CJC-1295 | GHRP-6= 100 mcg/day, CJC-1295= 150mcg/day | Subcutaneous(Abdomen) | 12 Weeks | Before Meal/Bed | Fat loss, Endurance, Strength | Injectable | |
| Semaglutide and AOD 9604 | Semaglutide= 0.25 mg/week, AOD 9604= 300 mcg/day | Subcutaneous (multipe skin sites) | 4 Weeks | Morning | Body Fat, Appetite, Muscle Mass | Injectable | |
| Epitalon and Thymalin | Epitalon= 5-10 mg/day, Thymalin= 10 mg/day | Subcutaneously, IV, IM | 2-3 Weeks | Morning | Strong Immune system, Anti-aging, Anti-inflammatory | Injectable |
All Peptide Guides
What Does Studied Dosage Mean?
The studied dose is the amount of a drug/agent which is proven most efficacious through multiple clinical trials. These trials help evaluate the optimum amount of a given drug that has maximum bioavailability and minimum to induce any negligible side effects.
It is therefore extremely crucial to abide by the correct dosage in order to mitigate the risk of developing any significant adverse effects at the expense of availing maximum benefits.
How Did We Find Typical Cycle Length?
Every peptide is cycled differently by various people. Cycle Length usually indicates the time period during which the drug starts showing its effects in the human body. For peptides, we referred to several clinical trials and user reviews to find the exact cycle length of each peptide.
How Did We Calculate the Timing of Dose?
There’s always a specific time for the intake of the drug alongside certain other precautions. With right timings, you can ensure a drug/supplement’s maximum bioavailability. In order to come up with the right time, we referred to user experiences/reviews as well as scientifically proven research.
What Does Use/Indication Mean?
Use/Indication highlights the purpose behind the intake of the chosen peptide. This is helpful for people to identify the most suitable peptide that would work for them and avoid the rest.
What Are the Recommended Forms to Use?
Recommended forms of peptides depend on the vendor as well as on its half life and bioavailability. Some drugs are only synthesized in injectable or capsule forms. Others can come in creams, skin patches, sprays etc.
While there may be different forms of peptide, it is usually the injectable form that provides the maximum bioavailability. However, injectable form is a strong contraindication in people with bleeding disorders or skin infections.
In general, the recommended form highly depends on the peptide that is being used.
Still Have Questions?
Drop us a comment down below and one of our expert coaches will respond to it, you can also email is at coaching@muscleandbrawn.com.
How does the math work when reconstituting a peptide with 5mg of two different peptides in the same vial? Example would be 5g of CJC-1295 and 5g of ipamorelin. if a dosage is say 20mcg of each individually, wht is a dosage of the mix?
did you ever get an answer? I have this same question…. Please let me know if you get an answer. Need to know how much to buy
I’m looking for the same answer.
If I have a 2mg bottle how much water total do I put into the vial?
That is AOD
How do I convert mcg to units.
It depends on the compound, there is no universal conversion
Where is the calculator?
Where is the calculator? I can’t find it
It’s fixed now!
How many Days worth does this reconstitute to? If I have a 5mg mass that I need to draw 300mcg doses daily, it tells me the volume to draw based on how many ML of water I use but it doesn’t tell me the quantity of doses that will yield
If you convert 5mg to mcg, it is 5000mcg. So divide 5000mcg by 300mcg.
What is considered “short term” with regards to storage? The vial I am reconstituting will probably last 8-10 weeks. Is that still short term?
It depends on the active compound within
I’m trying to reconstitute 5mg of trizeatide w 3ml of bacteriasc water.
Trying to figure out the mixture for 1 /wk dosage of .50mg.
How much water do I mix w the 5mg trizeatide? And how many dosages will I get out of one 5ml vial?
You just mentioned a 5mg vial and a 5ml vial. Those are not the same, please clarify
GM Amber. I am doing the same thing. Did you get a response
how long will one reconstituted vial of tirzepatide last in the frige
Peptides usually only last a couple of days / weeks once reconstituted
The calculator doesn’t work for custom doses
What is the dosage for tesomorelin
You can read more about that compound here: https://muscleandbrawn.com/peptides/tesamorelin/
How much bacteriostatic water is needed to reconstitute 5mg of Tirzepatide peptide to achieve a 100unit (10mg) dose?
I need this answer
… 5mg < 10mg. You could use 10,000L and 5 would still be smaller than 10
How much water do I add to 5mg Tirzepatide
I need to know your dose
How would you go about dosing a blend of tesamoraline, cjc 1295, and ipamoraline?
You can schedule a call with me here https://muscleandbrawn.com/coaching/
How Much Bacteriostatic Water do I add to 5mg of DSIp? I am a 57 year old male about 27LBS?
I reconstitute 2ml of Bacteriostatic into a 5mg peptide.
If I purchase a 10mg peptide and reconstitute with 4ml will it have the same effect?
Thanks
I am confused about how much water to use when mixing. Also do you keep it refrigerated between use?
You can use any amount of water, the dosage per unit just decreases. And yes, you have to refrigerate them
I don’t u derstand how every vial is advised to mix with 2ml. Rhe 10mg, 5mg and 2mg.
I have a 10 mg vial of TB500. How much Bariostatic water do I use to reconstitute? I will be using 7.5 mg / week in 2 equal doses on Tuesday and Thursday.