Puerariae Radix is a plant that has one particular constituent that is set to be the next superstar. It is called puerarin! Puerarin is an amazing nutrient that shows a 520% increase in growth hormone release (1) by stimulating the GHRH receptor. This ingredient was an amazing find but the key is the liquid "under the tongue" bioavailability which makes it biologically active. 520% increase in circulating GH is a HUGE increase and shows that this second pathway is very potent at releasing GH. We use only 98% pure puerarin complexed with cyclodextrin in our formula so it is ultra potent. Puerarin has also been shown to be an anti-aromatase, and a natural SERM, but this is not the main effect, compared to the GHRH stimulation, just a side benefit (3, 4). Finally, this wonder ingredient is a natural phosphoinositol 3 kinase (PI3K) stimulator which is the same anabolic pathway stimulated by IGF-1 and insulin to load the muscle with glycogen. I have to say, even I am impressed by this amazing material. GHRH stimulation, Aromatase Inhibition, natural SERM activity AND PI3K activity! Jackpot!
These two ultra potent pathways are what give GHenerate the amazing body recomp, appetite increasing effects that were experienced by our testers and make it the most potent GH secreting agent ever produced. One tester gained 8lbs on GHenerate alone, which is unheard of for a GH product. There is nothing on the market like GHenerate!
What about IGH-1? Luckily IGH-1, our GH secretagogue works in a totally different manner, which is to reduce somatostatin, meaning it will enhance and increase the potential of GHenerate. Somatostatin is the natural hormone your body produces to reduce GH production, so blocking this is a very good thing to do with GHenerate.
1) Horm Metab Res. 2004 Feb;36(2):86-91. Induction of growth hormone release by Pueraria thunbergiana BENTH. Jung DY, Ha H, Kim C. Drug Research and Development Team, Korea Institute of Oriental Medicine, 129-11 Chungdam-dong, Kangnam-ku, Seoul 135-100, Korea.
Puerariae Radix (PR), Puerariae Flos (PF), and Puerariae Surculus (PS) as well as their constituents were tested for induction of rat growth hormone (rGH) release by both rat pituitary cell culture and in vivo experimentation in order to develop them to novel drugs. Through a calibration curve of the rGH released by addition of rat growth hormone-releasing hormone (rGHRH) to rat pituitary cells, the 70 % ethanol extracts of PR and PS increased rGH release by about 1.6 and 1.7 times as high, respectively, as the control group (264.6 +/- 13.6 pM). However, each puerarin type as a representative constituent of PR in Korea Pharmacopeia (KP) and tectorigenin and an important ingredient of PF were twice as effective as in the control group. The acid hydrolysate of Puerariae Surculus (HPS) increased rGH release concentration-dependently, and its EC (50) was approximately 10.4 microg/ml. The T (max) value for rGH after injection of 20 microg/kg of rGHRH was 10 - 30 min, while the C (max) value was increased by approximately 12-fold compared to the control group (198.2 +/- 25.0 pM) and the AUC (0 - 45) was increased to 10 times the level of the control group (10,840.9 +/- 845.5 min. pM). On the other hand, T (max) for the HPS was 60 min, while C (max) was increased approximately to 5.8 fold compared to control (244.1 +/- 36.4 pM). C (max) for puerarin was 1,028.6 +/- 502.7 pM, that is, approximately 5.2 times as high as the control level. However, tectorigenin (20 microg/kg) was of no statistical significance. Therefore, we suggest that the HPS and puerarin act either on GH secretagogue receptors or on GHRH receptor of somatotrophin as possible agonists or an inhibitor on somatostatin receptor to release rGH, respectively.
2) Peptides. 2006 Jul;27(7):1597-602. Epub 2006 Feb 17. Ghrelin in plants: what is the function of an appetite hormone in plants? Aydin S Department of Biochemistry and Clinical Biochemistry, School of Medicine, Firat University, Firat Medical Center, 23119 Elazig, Turkey.
In the present work, we provide compelling evidence for the expression of a ghrelin-like peptide hormone that has only been associated with animals, in various plant tissues. Ghrelin, the appetite stimulating hormone, has been identified from a number of different species including humans, rat, pig, mouse, gerbil, eel, goldfish, bullfrog and chicken. The study here was conducted using an immunohistochemistry assay to screen whether plants have any ghrelin immunoreactivity. In this respect, Prunus x domestica L. and Marus alba were examined. Immunohistochemistry results showed that there is a strong human ghrelin immunoreactivity substance in the parenchyma cells of these plants. This was entirely unexpected since this hormone was considered to be present solely in animals. Thus, this study is the first to report the presence of a peptide with ghrelin-like activity in plants, a finding that has only been observed in the animal kingdom. RIA analysis confirmed that these plants contain significant amounts of this substance. Furthermore, reverse-phase HPLC analyses of plant extracts showed an elution characteristic of the peptide identical to that of human ghrelin. In general, fruit from both plants had higher levels of the peptide than the vegetative parts.
3) Food Chem Toxicol. 2008 Dec;46(12):3671-6. Epub 2008 Sep 23. Decreased expression of aromatase in the Ishikawa and RL95-2 cells by the isoflavone, puerarin, is associated with inhibition of c-jun expression and AP-1 activity. Li Y, Chen H, Yang S, Xie G. Cancer Hospital of Tianjin, Tianjin Medical University, Ti-Yuan-Bei, Tianjin 330060, China. email@example.com
4) Toxicol Appl Pharmacol. 2008 Dec 15;233(3):371-81. Epub 2008 Sep 19. Mechanism of phytoestrogen puerarin-mediated cytoprotection following oxidative injury: estrogen receptor-dependent up-regulation of PI3K/Akt and HO-1. Hwang YP, Jeong HG.